In vitro allergy diagnosis: comparison of a new method of fully automated determination of specific IgE, using Immulite 2000 compared with UniCAP 100 [ImmunoCAP 100]

Vignati G, Pastori E, Portalupi S, Temporiti R. Eur Ann Allergy Clin Immunol.
2003 Oct;35(8):285-94.

Summary
The availability of a method for the fully automated determination of specific IgE (sIgE) based on a system which has normally been used for conventional determination by immunochemical methods (Immulite 2000; DPC Los Angeles, CA), and which has been used for this purpose for several years in our organisation, led us to verify the analytical quality of the proposed method, leaving aside purely organisational aspects which we have discussed elsewhere, by carrying out appropriate testing in accordance with a sufficiently comprehensive and detailed protocol. We chose as our control method the procedure historically used in our laboratory and generally considered valid for the in-vitro determination of sIgE (UniCAP 100 [ImmunoCAP 100]; Pharmacia Diagnostics AB, Uppsala, Sweden).

Request full article